There is no difference on how many Master and Slave Nodes your Kubernetes cluster has if you want to install Istio.

You can follow the instructions from this link

Briefly, you need to:

1. Download Istio release
2. Install Istio’s Custom Resource Definitions using kubectl apply -f install/kubernetes/helm/istio/templates/crds.yaml from that release
3. Install Istio components using one of options:
   • without mutual TLS authentication between sidecars using kubectl apply -f install/kubernetes/istio-demo.yaml
   • with default mutual TLS authentication kubectl apply -f install/kubernetes/istio-demo-auth.yaml
   • Render Kubernetes manifest with Helm and deploy with kubectl
   • Use Helm and Tiller to manage the Istio deployment

For auto injection, you need to install istio-sidecar-injector component and add istio-injection=enabled label for a Namespace in which you want it to work. Example of commands:

kubectl label namespace <namespace> istio-injection=enabled
kubectl create -n <namespace> -f <your-app-spec>.yaml